How to Perform DNA tests- PCR and Gel Electrophoresis
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Description
In this lesson, students learn how to make and read gel electrophoresis tests in an interactive, inquiry inspired way. The steps of the lesson are listed below.
1. Students watch a video about how PCR is performed and describe it.
2. Students perform "PCR" by writing in the complementary strand to each side of a split DNA.
3. Students use "restriction enzymes" to cut a DNA strand and count the number of nucleotides in each piece.
4. Students draw "bands" on the proper spot on a gel based on their results in step 3.
5. Students examine gel results from a crime scene, from a paternity test, and from an evolutionary study. In each case, students are told what the "answer" is and have to explain how the gel results show this answer. In this way, they discover how to read gel electrophoresis results on their own rather than being told how to.
6. Students practice their skills with five practice problems.
A digital version of this assignment is also available here https://www.teacherspayteachers.com/My-Products/search:gel%20electrophoresis
